Immune profiling
Characterize T-cell and B-cell receptor repertoires across samples, experimental conditions and time points.
Analyses are tailored to your experimental design, data quality and research question. The workflow and deliverables are agreed following project review.
Analysis modules
V(D)J reconstruction and annotation, with protocol-specific quality control.
Clonotype definition, productive sequence filtering and chain-pairing assessment.
CDR3 length, sequence composition and V/J gene usage.
Repertoire diversity, clonality and sequencing-depth sensitivity analysis.
Repertoire overlap, shared clonotypes and between-group comparisons.
Longitudinal clonal expansion and contraction.
Single-cell receptor integration with cell states and phenotypes.
Exploratory similarity to curated antigen-associated receptor references.
BCR somatic hypermutation, isotype distributions and clonal lineage reconstruction where supported.
Data and metadata
AIRR or clonotype tables; compatible 10x V(D)J outputs; FASTQ where reconstruction is in scope. Sample metadata, library protocol and chain information are required.
Proposed deliverables
Annotated repertoire tables; diversity and overlap figures; expansion summaries; BCR lineage views where applicable; documented definitions and statistical contrasts.
Interpretation and feasibility
Sequence similarity or a database match is not experimental proof of antigen specificity. Bulk RNA-derived repertoires are not interchangeable with dedicated AIRR-seq. BCR affinity is not established from lineage or mutation analysis.
Methods and references
Methods, reference resources and software are selected for each project after protocol, feasibility and licence review. Applicable versions, references and interpretation limits are documented in the agreed workflow.